Assessment Of The Anti-Fungal Activity Of Trichoderma harzianum Against Seed-Borne Chickpea Ascochyta Blight Under Field Conditions

Qasim A Marzani; Kamalaldeen M. Fatah

doi:10.21271/ZJPAS.37.3.10

Authors

Qasim A Marzani Department of Plant Protection, College of Agricultural Engineering Sciences, Salahaddin University-Erbil, Erbil, Kurdistan Region , Iraq.
Kamalaldeen M. Fatah Department of Plant Protection, College of Agricultural Engineering Sciences, Salahaddin University-Erbil, Erbil, Kurdistan Region , Iraq.

DOI:

https://doi.org/10.21271/ZJPAS.37.3.10

Keywords:

: Chickpea, Blight, Ascochyta, Biocontrol.

Abstract

Ascochyta rabiei (Pass.) Labr., the fungal pathogen that causes ascochyta blight, is a serious global danger to chickpea crops, especially in the Mediterranean and other colder, wetter climates. This foliar disease can cause losses of up to 50% and is particularly damaging in humid and rainy environments. Severe outbreaks frequently result in total crop destruction. Chemical control, despite providing quick and efficient intervention, presents challenges such as environmental contamination and the evolution of fungicide resistance due to the heterothallic nature of the fungus. This study aimed to explore the use of antagonistic fungi as a safer alternative for managing Ascochyta blight damage. In a field trial, the commercial product Biocont-T, containing Trichoderma harzianum, was applied as a pre-planting treatment and mixed with peat moss at various rates. The experiment followed a randomized complete block design (RCBD) and was replicated three times. Results indicated that the most effective treatment inhibiting the disease (55.55%) involved a peat-based soil inoculation mixture containing 20.8 kg/ha of Biocont-T and 2083 kg/ha of peat moss (T3). This was followed by T1 (20.8 kg/ha of Biocont-T), which inhibited the disease by 52.77%, T2 (41.66 kg/ha of Biocont-T) with a 41.66% inhibition rate, and T6 (1041.66 kg/ha of pre-inoculated peat moss with the bioagent for one week), also with a 41.66% inhibition rate. In conclusion, while using T. harzianum as a biocontrol agent did not completely eradicate the disease, it proved an effective method with no adverse environmental effects.

References

ADNAN, M., ISLAM, W., SHABBIR, A., KHAN, K. A., GHRAMH, H. A., HUANG, Z., CHEN, H. Y. and LU, G.-D. 2019. Plant defense against fungal pathogens by antagonistic fungi with Trichoderma in focus. Microbial pathogenesis, 129, 7-18.

AHMAD, S., KHAN, M. A., AHMAD, I., IQBAL, Z., ASHRAF, E., ATIQ, M., ALI, Y. and NASEER, S. 2021. Efficacy of fungicides, plant extracts and biocontrol agents against Ascochyta blight (Ascochyta rabiei) of chickpea (Cicer arietinum L.) under field conditions. Plant Science Today, 8, 255-262.

ALABOUVETTE, C., LEMANCEAU, P. and STEINBERG, C. 1993. Recent advances in the biological control of Fusarium wilts. Pesticide Science, 37, 365-373.

ARMSTRONG, C., CHONGO, G., GOSSEN, B. and DUCZEK, L. 2001. Mating type distribution and incidence of the teleomorph of Ascochyta rabiei (Didymella rabiei) in Canada. Canadian Journal of Plant Pathology, 23, 110-113.

ASAD, S. A. 2022. Mechanisms of action and biocontrol potential of Trichoderma against fungal plant diseases-A review. Ecological Complexity, 49, 100978.

ATANASOVA, L., CROM, S. L., GRUBER, S., COULPIER, F., SEIDL-SEIBOTH, V., KUBICEK, C. P. and DRUZHININA, I. S. 2013. Comparative transcriptomics reveals different strategies of Trichoderma mycoparasitism. BMC genomics, 14, 1-15.

BENZOHRA, I., BENDAHMANE, B., LABDI, M. and YOUCEF BNEKADA, M. 2011. In vitro biocontrol using the antagonist Trichoderma harzianum against the Algerian isolates of Ascochyta rabiei (Pass.) Labr., the agent of Ascochyta Blight in Chickpea (Cicer arietinum L.). International Journal of Microbiological Research, 2, 124-128.

BENZOHRA, I. E., BENDAHMANE, B. S., BENKADA, M. Y. and LABDI, M. 2014. Evaluation of wild Cicer species accessions for resistance to three pathotypes of Ascochyta rabiei (Pass.) Labr. in Algeria. African Journal of Microbiology Research, 8, 2022-2029.

BENZOHRA, I. E., BENDAHMANE, B. S., BENKADA, M. Y., MÉGATELI, M. and BELAIDI, H. 2020. Use of Three Synthetic Fungicides to Reduce the Incidence of Ascochyta Blight (Ascochyta rabiei) in Chickpea (Cicer arietinum L.): A Susceptible Cultivars Case. Indian Journal of Agricultural Research, 54.

BOWNESS, R., GOSSEN, B. D., CHANG, K.-F., GOSWANI, R., WILLENBORG, C. J., HOLTZ, M. and STRELKOV, S. E. 2016. Sensitivity of Mycosphaerella pinodes to pyraclostrobin fungicide. Plant Disease, 100, 192-199.

BRENT, K. J. and HOLLOMAN, D. W. 2007. Fungicide resistance: The assessment of risk. . 2nd ed. Fungicide Resistance Action Committee (FRAC). Crop Life International, Brussels, Belgium.

CAI, F., CHEN, W., WEI, Z., PANG, G., LI, R., RAN, W., SHEN, Q. and SOIL 2015. Colonization of Trichoderma harzianum strain SQR-T037 on tomato roots and its relationship to plant growth, nutrient availability and soil microflora. Plant Soil, 388, 337-350.

CARLILE, W., RAVIV, M. and PRASAD, M. 2019. Organic soilless media components. Soilless Culture, 303-378.

CHANG, K., AHMED, H., HWANG, S., GOSSEN, B., STRELKOV, S., BLADE, S. and TURNBULL, G. 2007. Sensitivity of field populations of Ascochyta rabiei to chlorothalonil, mancozeb and pyraclostrobin fungicides and effect of strobilurin fungicides on the progress of ascochyta blight of chickpea. Canadian journal of plant science, 87, 937-944.

CHONGO, G., GOSSEN, B., BUCHWALDT, L., ADHIKARI, T. and RIMMER, S. 2004. Genetic diversity of Ascochyta rabiei in Canada. Plant disease, 88, 4-10.

DIAPARI, M., SINDHU, A., BETT, K., DEOKAR, A., WARKENTIN, T. D. and TAR’AN, B. 2014. Genetic diversity and association mapping of iron and zinc concentrations in chickpea (Cicer arietinum L.). Genome, 57, 459-468.

DOS SANTOS, M. F., DOS SANTOS, L. E., DA COSTA, D. L., VIEIRA, T. A. and LUSTOSA, D. C. 2020. Trichoderma spp. on treatment of Handroanthus serratifolius seeds: effect on seedling germination and development. Heliyon, 6 (6).

EL-SAADONY, M. T., SAAD, A. M., SOLIMAN, S. M., SALEM, H. M., AHMED, A. I., MAHMOOD, M., EL-TAHAN, A. M., EBRAHIM, A. A., EL-MAGEED, A. and TAIA, A. 2022. Plant growth-promoting microorganisms as biocontrol agents of plant diseases: Mechanisms, challenges and future perspectives. Frontiers in plant science, 13, 923880.

ELIA, Y. I. and MARZANI, Q. A. 2005. Effect Of Date Of Sowing On Chickpea Blight Caused By Ascochyta rabiei. Zanco Journal for Pure and Applied Sciences, 17, 79-87.

GAONA, L., BEDMAR, F., GIANELLI, V., FABERI, A. and ANGELINI, H. 2019. Estimating the risk of groundwater contamination and environmental impact of pesticides in an agricultural basin in Argentina. International Journal of Environmental Science and Technology, 16, 6657-6670.

HAJIEGHRARI, B., TORABI-GIGLOU, M., MOHAMMADI, M. R. and DAVARI, M. 2008. Biological potantial of some Iranian Trichoderma isolates in the control of soil borne plant pathogenic fungi. African Journal of Biotechnology, 7.

HOITINK, H. and BOEHM, M. 1999. Biocontrol within the context of soil microbial communities: a substrate-dependent phenomenon. Annual review of phytopathology, 37, 427-446.

HORBACH, R., NAVARRO-QUESADA, A. R., KNOGGE, W. and DEISING, H. B. 2011. When and how to kill a plant cell: infection strategies of plant pathogenic fungi. Journal of plant physiology, 168, 51-62.

IZURDIAGA, D., SÁNCHEZ-LÓPEZ, Á. M., SAN MILLAN, A. F. and POVEDA, J. 2023. Cell-free filtrates from plant pathogens: Potential new sources of bioactive molecules to improve plant health. Crop Protection, 106477.

JADON, K. S., THIRUMALAISAMY, P., KUMAR, R. and MANAGEMENT 2020. Major seed-borne diseases in important pulses: Symptomatology, aetiology and economic importance. Seed-Borne Diseases of Agricultural Crops: Detection, Diagnosis, 469-542.

KAHRAMAN, A. and OZKAN, Z. 2016. Ascochyta blight of chickpea. Selcuk Journal of Agriculture and Food Sciences, 29, 62-66.

KANOUNI, H., TALEEI, A. and OKHOVAT, M. 2011. Ascochyta blight (Ascochyta rabiei (Pass.) Lab.) of chickpea (Cicer arietinum L.): breeding strategies for resistance. International Journal of Plant Breeding and Genetics, 5, 1-22.

KAREEM, H. J. and AL-ARAJI, A. M. 2017. Evaluation of Trichoderma harzianum biological control against Fusarium oxysporum f. sp. melongenae. Iraqi Journal of Science, 58, 2051-2060.

KHALIQ, I., FANNING, J., MELLOY, P., GALLOWAY, J., MOORE, K., BURRELL, D. and SPARKS, A. H. 2020. The role of conidia in the dispersal of Ascochyta rabiei. European Journal of Plant Pathology, 158, pp.911-924.

KOZICKI, M. and NIESŁOCHOWSKI, A. 2020. Materials Contamination and Indoor Air Pollution Caused by Tar Products and Fungicidal Impregnations: Intervention Research in 2014–2019. Sensors, 20, 4099.

KTHIRI, Z., JABEUR, M. B., MACHRAOUI, M., GARGOURI, S., HIBA, K. and HAMADA, W. 2020. Coating seeds with Trichoderma strains promotes plant growth and enhance the systemic resistance against Fusarium crown rot in durum wheat. Egyptian Journal of Biological Pest Control, 30, 1-10.

KÜÇÜK, Ç., KIVANÇ, M., KINACI, E. and KINACI, G. 2007. Efficacy of Trichoderma harzianum (Rifaii) on inhibition of ascochyta blight disease of chickpea. Annals of microbiology, 57, 665-668.

KUZMANOVSKA, B., RUSEVSKI, R., JANKULOVSKA, M. and ORESHKOVIKJ, K. B. 2018. Antagonistic activity of Trichoderma asperellum and Trichoderma harzianum against genetically diverse Botrytis cinerea isolates. Chilean journal of agricultural research, 78, 391-399.

LI, H., RODDA, M., GNANASAMBANDAM, A., AFTAB, M., REDDEN, R., HOBSON, K., ROSEWARNE, G., MATERNE, M., KAUR, S. and SLATER, A. T. 2015a. Breeding for biotic stress resistance in chickpea: progress and prospects. Euphytica, 204, 257-288.

LI, R.-X., CAI, F., PANG, G., SHEN, Q.-R., LI, R. and CHEN, W. 2015b. Solubilisation of phosphate and micronutrients by Trichoderma harzianum and its relationship with the promotion of tomato plant growth. PloS one, 10, e0130081.

MARZANI, Q. A. 2003. Epiphytotic and control of ascochyta blight of chickpea caused by Ascochyta rabiei (Pass.) Labr. in Erbil Province, MSc. thesis, Salahaddin University, Erbil.

MARZANI, Q. A., FATAH, K. M. and MUSTAFA, M. H. 2017. Field efficacy of the commercial formulation of the antagonistic Trichoderma harzianum on chickpea wilt caused by Fusarium oxysporum. Science Journal of University of Zakho, 5, 64-69.

MENGIST, Y., WASSIE, A. and ASRES, T. 2019. Response of chickpea varieties and sowing dates for the management of chickpea ascochyta blight (Ascochyta rabiei (Pass.) Disease at West Belesa District, Ethiopia. African Journal of Plant Science, 13, 231-238.

MICKINEY, H. H. 1923. Helminthosporium disease of wheat. Journal of Agricultural Research, 26, 195–218.

MISHRA, R., PANDEY, S., MISHRA, M., RATHORE, U. S., TRIPATHI, K. M., KUMAR, K. and BEYOND 2020. Trichoderma: An Effective and Potential Biocontrol Agent for Sustainable Management of Pulses Pathogens. Trichoderma: Agricultural Applications, 159-180.

MOHIDDIN, F. A., PADDER, S. A., BHAT, A. H., AHANGER, M. A., SHIKARI, A. B., WANI, S. H., BHAT, F. A., NABI, S. U., HAMID, A. and BHAT, N. A. 2021. Phylogeny and optimization of Trichoderma harzianum for chitinase production: Evaluation of their antifungal behaviour against the prominent soil borne phyto-pathogens of temperate India. Microorganisms, 9, 1962.

MORCUENDE, J., MARTÍN-GARCÍA, J., VELASCO, P., SÁNCHEZ-GÓMEZ, T., SANTAMARÍA, Ó., RODRÍGUEZ, V. M. and POVEDA, J. 2024. Effective biological control of chickpea rabies (Ascochyta rabiei) through systemic phytochemical defenses activation by Trichoderma roots colonization: From strain characterization to seed coating. J Biological Control, 193, 105530.

MOTAGI, B. N., RAO, M. L. and MATHAD, A. 2020. Integrated and Sustainable Management of Fungal Diseases of Chickpea: Current Status and Challenges. Management of Fungal Pathogens in Pulses. Springer.

MUEHLBAUER, F. and SINGH, K. (eds.) 1987. Genetics of Chickpea, 99-125, CAB International, Wallingford, Oxon, UK.

PANI, S., KUMAR, A., SHARMA, A. and SCIENCE, L. 2021. Trichoderma harzianum: An overview. Bulletin of Environment, Pharmacology, 10, 32-39.

PASCUAL, J. A., CEGLIE, F., TUZEL, Y., KOLLER, M., KOREN, A., HITCHINGS, R. and TITTARELLI, F. 2018. Organic substrate for transplant production in organic nurseries. A review. Agronomy for Sustainable Development, 38, 1-23.

PORTA-PUGLIA, A., CRINO, P. and MOSCONI, C. 1996. 2293881. Variability in virulence to chickpea of an Italian population of Ascochyta rabiei. Plant Disease, 80, 39-41.

RAJAKUMAR, E., AGGARWAL, R. and SINGH, B. 2005. Fungal antagonists for the biological control of Ascochyta blight of chickpea. Acta Phytopathologica et Entomologica Hungarica, 40, 35-42.

REDDY, M. and SINGH, K. 1990. Relationship between ascochyta blight severity and yield loss in chickpea and identification of resistant lines. Phytopathologia mediterranea, 32-38.

SHARFUDDIN, C., MOHANKA, R. and RESEARCH, P. 2012. In vitro antagonism of indigenous Trichoderma isolates against phytopathogen causing wilt of lentil. International Journal of Life Science, 2, 195-202.

SHARMA, A., ARYA, S. K., SINGH, J., KAPOOR, B., BHATTI, J. S., SUTTEE, A. and SINGH, G. 2023. Prospects of chitinase in sustainable farming and modern biotechnology: an update on recent progress and challenges. Biotechnology Genetic Engineering Reviews, 1-31.

SHARMA, M. and GHOSH, R. 2016. An update on genetic resistance of chickpea to ascochyta blight. Agronomy, 6, 18.

SHARMA, P., KUMAR, V., RAMESH, R., SARAVANAN, K., DEEP, S., SHARMA, M., MAHESH, S. and DINESH, S. 2011. Biocontrol genes from Trichoderma species: a review. African Journal of Biotechnology, 10, 19898-19907.

STEPHENS, A., LOMBARDI, M., COGAN, N. O., FORSTER, J. W., HOBSON, K., MATERNE, M. and KAUR, S. 2014. Genetic marker discovery, intraspecific linkage map construction and quantitative trait locus analysis of Ascochyta blight resistance in chickpea (Cicer arietinum L.). Molecular breeding, 33, 297-313.

SUBASH, N., MEENAKSHISUNDARAM, M., UNNAMALAI, N., SASIKUMAR, C. and SCIENCES, A. 2013. In vitro evaluation of different strains of Trichoderma harzianum as biocontrol agents of chilli. International Journal of Biology, Pharmacy, 2, 495-500.

SUTHAR, K., PATEL, R., SINGH, D. and KHUNT, M. 2017. Efficacy of Bacillus subtilis isolate K18 against chickpea wilt Fusarium oxysporum F. Sp. ciceri. International journal of pure and applied bioscience, 5, 838-843.

TYŚKIEWICZ, R., NOWAK, A., OZIMEK, E. and JAROSZUK-ŚCISEŁ, J. 2022. Trichoderma: The current status of its application in agriculture for the biocontrol of fungal phytopathogens and stimulation of plant growth. International Journal of Molecular Sciences, 23, 2329.

VAN DER MAESEN, L. 1987. Origin, history and taxonomy of chickpea. In: M. C. SAXENA, A. K. B. S. (ed.) The chickpea (pp 11 - 34). Wallingford: C.A.B. International.

VANDANA, U. K., SINGHA, P. B., CHAKRABORTHY, S. and MAZUMDER, P. 2020. Integrated Fungal Foliar Diseases of Arid Legumes: Challenges and Strategies of Their Management in Rain-Fed Areas. Management of Fungal Pathogens in Pulses. Springer.

VIR, S. and GREWAL, J. 1974. Physiologic specialization in Ascochyta rabiei the causal organism of gram blight. Indian Phytopathology, 27, 355 - 360.

WISE, K., BRADLEY, C., PASCHE, J. and GUDMESTAD, N. 2009. Resistance to QoI fungicides in Ascochyta rabiei from chickpea in the Northern Great Plains. Plant Disease, 93, 528-536.

WISE, K. A., BRADLEY, C. A., MARKELL, S., PASCHE, J., DELGADO, J. A., GOSWAMI, R. S. and GUDMESTAD, N. C. 2011. Sensitivity of Ascochyta rabiei populations to prothioconazole and thiabendazole. Crop Protection, 30, 1000-1005.

WOO, S. L., RUOCCO, M., VINALE, F., NIGRO, M., MARRA, R., LOMBARDI, N., PASCALE, A., LANZUISE, S., MANGANIELLO, G. and LORITO, M. 2014. Trichoderma-based products and their widespread use in agriculture. The Open Mycology Journal, 8, 71 - 126.

YASSIN, M. T., MOSTAFA, A. A.-F. and AL-ASKAR, A. A. 2021. In vitro antagonistic activity of Trichoderma harzianum and T. viride strains compared to carbendazim fungicide against the fungal phytopathogens of Sorghum bicolor (L.) Moench. Egyptian Journal of Biological Pest Control, 31, 1-9.

YIN, Y., MIAO, J., SHAO, W., LIU, X., ZHAO, Y. and MA, Z. 2023. Fungicide resistance: Progress in understanding mechanism, monitoring, and management. Phytopathology, 113, 707-718.

ZEILINGER, S., GRUBER, S., BANSAL, R. and MUKHERJEE, P. K. 2016. Secondary metabolism in Trichoderma–chemistry meets genomics. Fungal biology reviews, 30, 74-90.