Study of the phytochemical diversity of segregated Rhus coriaria pericarp and seeds oil and their antifungal and antibacterial activity

Dotsha Jaleel Raheem; Hero Sabah Rahman; Nareen Qadr FaqeAbdulla; Akhter    Ahmed Ahmed

doi:10.21271/ZJPAS.37.1.1

Authors

Dotsha Jaleel Raheem Department of Chemistry, College of Science, Salahaddin University-Erbil, Erbil, Kurdistan Region, Iraq
Hero Sabah Rahman Department of Chemistry, College of Science, Salahaddin University-Erbil, Erbil, Kurdistan Region, Iraq
Nareen Qadr FaqeAbdulla Department of Biology, College of Science, Salahaddin University-Erbil, Erbil, Kurdistan Region, Iraq
Akhter Ahmed Ahmed Department of Biology, College of Science, Salahaddin University-Erbil, Erbil, Kurdistan Region, Iraq

DOI:

https://doi.org/10.21271/ZJPAS.37.1.1

Keywords:

Caryophyllene, DPPH, Pericarp, Seed oil, Sumac

Abstract

Sumac fruits are used in food and as a remedy, but their seeds are often discarded as waste despite making up a large proportion of the fruit’s weight. This research therefore examines the chemical composition and biological activities of oils from sumac pericarp and seeds in an attempt to find potential uses for the seeds in comparison to the long-used pericarp. Both oils were found to contain alkanes and fatty acids. The pericarp oil also contained caryophyllenes. Fatty acid methyl ester analysis revealed oleic, linoleic, palmitic, and stearic acids as major components in both parts. Pericarp oil contained more saturated fatty acids, while seed oil showed higher content of unsaturated fatty acids. The pericarp oil also contained greater amounts of total phenols, β-carotene and exhibited higher radical scavenging activity. Elemental analysis found K, P, Si, Cl, S, and Ca as major elements in both pericarp and seeds oil. Both oils showed moderate inhibition against Candida albicans. The pericarp oil strongly inhibited Escherichia coli and seed oil inhibited Acinetobacter baumannii. Moderate activity was observed against Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus by both pericarp and seeds oil. The findings suggest both oils have potential as nutritional and antimicrobial agents.

References

Abdul-Jalil, T.Z., 2020. Rhus coriaria (Sumac): A magical spice, in: Akram, M., Ahmad, R.S. (Eds.), Herbs and Spices. IntechOpen, (2020), London, UK, pp. 39–52.

Aboualigalehdari, E., Sadeghifard, N., Taherikalani, M., Zargoush, Z., Tahmasebi, Z., Badakhsh, B., Rostamzad, A., Ghafourian, S., Pakzad, I., (2016). Anti-biofilm Properties of Peganum harmala against Candida albicans. Osong Public Health Res Perspect 7, 116–118.

Ahmadian-Attari, M.M., Amini, M., Farsam, H., Amin, G., Fazeli, M.R., Esfahani, H.R.M., Jamalifar, H., Bairami, A., (2016). Isolation of Major Active Antibacterial Compounds of Sumac Fruit (Rhus coriaria L.). Int J Enteric Pathog 4, 1–5.

Aibinu, I., Adenipekun, T., Adelowotan, T., Ogunsanya, T., Odugbemi, T., (2007). Evaluation of the Antimicrobial Properties of Different Parts of Citrus Aurantifolia (Lime Fruit) as Used Locally. African Journal of Traditional, Complementary, and Alternative Medicines 4, 185.

Alsamri, H., Athamneh, K., Pintus, G., Eid, A.H., Iratni, R., (2021). Pharmacological and Antioxidant Activities of Rhus coriaria L. (Sumac). Antioxidants 10, 73.

Alves, E., Simoes, A., Domingues, M.R., (2021). Fruit seeds and their oils as promising sources of value-added lipids from agro-industrial byproducts: oil content, lipid composition, lipid analysis, biological activity and potential biotechnological applications. Crit Rev Food Sci Nutr 61, 1305–1339.

Ashraf, M.W., (2014). Levels of selected heavy metals in varieties of vegetable oils consumed in Kingdom of Saudi Arabia and health risk assessment of local population. Journal of the Chemical Society of Pakistan 36, 691–698.

Blasi, F., Cossignani, L., (2020). An Overview of Natural Extracts with Antioxidant Activity for the Improvement of the Oxidative Stability and Shelf Life of Edible Oils. Processes 2020, Vol. 8, Page 956 8, 956.

Domínguez, R., Pateiro, Mirian, Purriños, L., Munekata, P.E.S., Echegaray, N., Lorenzo, J.M., (2022). Introduction and classification of lipids, in: Lorenzo, J., Munekata, p, Pateiro, M, Barba, F., Domínguez, F. (Eds.), Food Lipids: Sources, Health Implications, and Future Trends. Academic Press, pp. 1–16.

Elagbar, Z.A., Shakya, A.K., Barhoumi, L.M., Al-Jaber, H.I., 2020. Phytochemical Diversity and Pharmacological Properties of Rhus coriaria. Chem Biodivers 17, e1900561.

FAO-WHO, (2023). Standards | CODEXALIMENTARIUS FAO-WHO [WWW Document]. Standards | CODEXALIMENTARIUS FAO-WHO, CXS 19-1981, CCFO. URL https://www.fao.org/fao-who-codexalimentarius/codex-texts/list-standards/en/ (accessed 8.9.24).

Francomano, F., Caruso, A., Barbarossa, A., Fazio, A., Torre, C. La, Ceramella, J., Mallamaci, R., Saturnino, C., Iacopetta, D., Sinicropi, M.S., (2019). β-Caryophyllene: A Sesquiterpene with Countless Biological Properties. Applied Sciences 9, 5420.

González-Torres, P., Puentes, J.G., Moya, A.J., La Rubia, M.D., (2023). Comparative Study of the Presence of Heavy Metals in Edible Vegetable Oils. Applied Sciences 13, 3020–3049.

Jabbar, A.A., Abdulrahman, K.K., Abdulsamad, P., Mojarrad, S., Mehmetçik, G., Sardar, A.S., (2023). Phytochemical profile, Antioxidant, Enzyme inhibitory and acute toxicity activity of Astragalus bruguieri. Baghdad Science Journal 20, 0157–0157.

Karadaş, Ö., Yılmaz, İ., Geçgel, Ü., (2022). Determination of physicochemical properties of irradiated sumac (Rhus coriaria L.) fruit oils. Radiation Physics and Chemistry 198, 110210.

Karadaş, Ö., Yılmaz, İ.C., Geçgel, Ü., (2020). Properties of Sumac Plant and Its Importance in Nutrition. International Journal of Innovative Approaches in Agricultural Research 4, 378–383.

Khalilpour, S., (2019). VALIDATION OF PLANTS TRADITIONALLY USED FOR SKIN INFLAMMATION.

Khoshkharam, M., Shahrajabian, M.H., Sun, W., Cheng, Q., (2020). Sumac (Rhus coriaria L.) a spice and medicinal plant – a mini review. Amazonian Journal of Plant Research 4, 517–523.

Kossah, R., Nsabimana, C., Zhao, J., Chen, H., Tian, F., Zhang, H., Chen, W., (2009). Comparative study on the chemical composition of Syrian sumac (Rhus coriaria L.) and Chinese sumac (Rhus typhina L.) fruits. Pakistan Journal of Nutrition 8, 1570–1574.

Liput, K.P., Lepczyński, A., Ogłuszka, M., Nawrocka, A., Poławska, E., Grzesiak, A., Ślaska, B., Pareek, C.S., Czarnik, U., Pierzchała, M., (2021). Effects of Dietary n–3 and n–6 Polyunsaturated Fatty Acids in Inflammation and Cancerogenesis. International Journal of Molecular Sciences 22, 6965.

Manzoor, J., Sharma, M., Wani, K.A., (2018). Heavy metals in vegetables and their impact on the nutrient quality of vegetables: A review. J Plant Nutr 41, 1744–1763.

Matthaus, B., Özcan, M.M., (2015). Fatty acid composition, tocopherol, and sterol contents of sumac (Rhus coriaria L.) fruit oils. European Journal of Lipid Science and Technology 117, 1301–1302.

Molole, G.J., Gure, A., Abdissa, N., (2022). Determination of total phenolic content and antioxidant activity of Commiphora mollis (Oliv.) Engl. resin. BMC Chem 16, 1–11.

Morshedloo, M.R., Fereydouni, S., Ahmadi, H., Hassanpouraghdam, M.B., Aghaee, A., Vojodi Mehrabani, L., Maggi, F., (2022). Natural diversity in fatty acids profiles and antioxidant properties of sumac fruits (Rhus coriaria L.): Selection of preferable populations for food industries. Food Chem 374, 131757.

Nayebpour, N., Asadi-Gharneh, H.A., (2019). Variability of Fatty Acids Composition of Wild Sumac (Rhus coriaria L.) Fruit. Journal of Medicinal Plants 18, 118–129.

Ornella, T., Flore, T., Tiencheu, B., Tenyang, N., Ashu, A., Marie, M., Ufuan, A., (2022). Chemical and Antibacterial Properties of Lipids Extracted from Some Plant Seeds and Fruits Commonly Used in Cosmetics. American Journal of Food Science and Technology 10, 10–19.

Petropoulos, S.A., Fernandes, Â., Calhelha, R.C., Rouphael, Y., Petrović, J., Soković, M., Ferreira, I.C.F.R., Barros, L., (2021). Antimicrobial Properties, Cytotoxic Effects, and Fatty Acids Composition of Vegetable Oils from Purslane, Linseed, Luffa, and Pumpkin Seeds. Applied Sciences 11, 5738.

Rashid, T.S., Awla, H.K., Sijam, K., (2018). Antifungal effects of Rhus coriaria L. fruit extracts against tomato anthracnose caused by Colletotrichum acutatum. Ind Crops Prod 113, 391–397.

Rashid, T.S., Sijam, K., Kadir, J., Saud, H.M., Awla, H.K., Zulperi, D., Hata, E.M., (2016). Screening for active compounds in Rhus coriaria L. crude extract that inhibit the growth of Pseudomonas syringae and Ralstonia solanacearum. Indian J Agric Res 50, 15–21.

Rayne, S., Mazza, G., (2007). Biological Activities of Extracts from Sumac (Rhus spp.): A Review. Plant foods for human nutrition 62, 165–175.

Rodriguez-Amaya, D.B., (2001). A guide to carotenoid analysis in foods, Washington, D. C. ILSI Press.

Shabbir, A., (2012). RHUS CORIARIA LINN, A PLANT OF MEDICINAL, NUTRITIONAL AND INDUSTRIAL IMPORTANCE: A REVIEW. The Journal of Animal & Plant Sciences 22, 505–512.

Shahrivari, S., Zeebaree, S.M.S., Alizadeh-Salteh, S., Feizy, H.S., Morshedloo, M.R., (2024). Phytochemical variations antioxidant, and antibacterial activities among zebaria sumac (Rhus coriaria var. zebaria) populations in Iraq. Sci Rep 14.

Srinivasan, D., Nathan, S., Suresh, T., Lakshmana Perumalsamy, P., (2001). Antimicrobial activity of certain Indian medicinal plants used in folkloric medicine. J Ethnopharmacol 74, 217–220.

Suárez, M., Gual-Grau, A., Ávila-Román, J., Torres-Fuentes, C., Mulero, M., Aragonès, G., Bravo, F.I., Muguerza, B., (2021). Oils and Oilseeds in the Nutraceutical and Functional Food Industries, in: Lafarga, T., Bobo, G., Aguilo-Aguayo, I. (Eds.), Oil and Oilseed Processing: Opportunities and Challenges. John Wiley & Sons, Ltd, 2021, pp. 219–243.

Valgas, C., De Souza, S.M., Smânia, E.F.A., Smânia, A., (2007). Screening methods to determine antibacterial activity of natural products. Brazilian Journal of Microbiology 38, 369–380.

Yilmaz, G., Ekşi, G., Demirci, B., Demirci, F., (2020). Chemical characterization of the fatty acid compositions and antimicrobial activity of sumac (Rhus coriaria L.) fruits, growing naturally in Turkey and sold in herbalist markets. Ankara Universitesi Eczacilik Fakultesi Dergisi 44, 61–69.

Zhukov, A. V., Shumskaya, M., 2020. Very-long-chain fatty acids (VLCFAs) in plant response to stress. Functional Plant Biology 47, 695–703.