Assessment of Risk Behaviors and Toxic Heavy Metals Exposure of Car Dye Workers in Repairing Services in Erbil City, Kurdistan Region, Iraq

Hawraz Sami Khalid; Shokhan Sherzad Hamadamin; Hanan Salm Salih

doi:10.21271/ZJPAS.32.6.1

Authors

Hawraz Sami Khalid Department of Chemistry, College of Education, Salahaddin University-Erbil, Erbil City, Kurdistan Region, Iraq
Shokhan Sherzad Hamadamin Department of Chemistry, College of Education, Salahaddin University-Erbil, Erbil City, Kurdistan Region, Iraq
Hanan Salm Salih Department of Chemistry, College of Education, Salahaddin University-Erbil, Erbil City, Kurdistan Region, Iraq

DOI:

https://doi.org/10.21271/ZJPAS.32.6.1

Keywords:

Heavy metals, Blood, Painters, ICP-OES, Erbil City

Abstract

The main objective of this study was to determine blood cadmium (BCd) and blood lead (BPb) contents for 16 car dye workers in repairing service and compared to those of 16 controls (non-occupational exposed subjects) in Erbil City, Kurdistan Region, Iraq. Strong wet acid digestion method was applied to digest whole blood samples using a mixture H₂O₂ (50% v/v) and HNO₃ (65% v/v) with a volume ration (1:2). Inductively coupled plasma - optical emission spectrometer (ICP-OES) was used to determine these toxic metals in collected whole blood samples. The recorded mean of BCd and BPb contents in subjected painters were higher levels compared to the control group. The mean ± standard deviation (SD) contents of BCd and BPb of painters were 2.93±2.35 μg/L and 32.19±16.95 μg/L, respectively. The mean ± SD contents of BCd and BPb of controls were 0.870±0.34 μg/L and 22.46±12.55 μg/L, respectively. A set of formal investigation questionnaire was performed to know participants history profile, smoking status, clinical profile, the risk behaviors at the workplace, and occurrence symptoms. The results showed that the percentages of occurrence of most investigated symptoms (especially respiratory and musculoskeletal) system on the experimental painters were significantly (p-value = 0.016) higher than those symptoms of the control group. The collected data on risk behaviors confirmed that personal protection equipment (PPE) had not been concerned by most of the experimental painters. Duration of work (years), and the use of PPE (wearing a respirator) were noticeably associated with BCd and BPb levels of painters.

References

AFRIDI, H. I., KAZI, T. G., KAZI, A. G., SHAH, F., WADHWA, S. K., KOLACHI, N. F., SHAH, A. Q., BAIG, J. A. & KAZI, N. 2011. Levels of arsenic, cadmium, lead, manganese and zinc in biological samples of paralysed steel mill workers with related to controls. Biological trace element research, 144, 164-182.

AKINCI, I., TUTKUN, E., TURKSOY, V., YILMAZ, H., YUKSEL, B., KAYAALTI, Z., SOYLEMEZOGLU, T., YILMAZ, H. & ABUSOGLU, S. 2016. Toxic metal and essential trace element levels of blood donors. J Clin Anal Med, 7, 816-9.

AKINLOYE, O., OGUNLEYE, K. & OGUNTIBEJU, O. O. 2010. Cadmium, lead, arsenic and selenium levels in patients with type 2 diabetes mellitus. African Journal of Biotechnology, 9, 5189-5195.

AL-ATTAR, M. S. 2016. Effects of Nickel and Cadmium Contaminated Fish Meat on Chromosomal Aberrations and Sperm Morphology of Swiss Albino Mice. ZANCO Journal of Pure and Applied Sciences, 28.

ALIOMRANI, M., SAHRAIAN, M. A., SHIRKHANLOO, H., SHARIFZADEH, M., KHOSHAYAND, M. R. & GHAHREMANI, M. H. 2016. Blood concentrations of cadmium and lead in multiple sclerosis patients from Iran. Iranian journal of pharmaceutical research: IJPR, 15, 825.

ALROBAIAN, M. & ARIDA, H. 2019. Assessment of Heavy and Toxic Metals in the Blood and Hair of Saudi Arabia Smokers Using Modern Analytical Techniques. International journal of analytical chemistry, 2019, 1-8.

AMETEK. FHS22. Spectro ICP Report; Analysis of Aqueous Solutions by ICP-OES with Radial Plasma Observation. Spectro Arcos, Spectro Analytical Instruments GmbH Germany [Online]. Germany. Available: https://extranet.spectro.com/-/media/31793ADA-B987-4D37-B597-04AFB66C7C22.pdf [Accessed 20 Jan 2020].

AMIN, J. K. M., JALAL, S. S. & JARJEES, F. Z. 2017. The Elemental Composition of Atmospheric Particles and Dust Fall Rate in Erbil Governorate. ZANCO Journal of Pure and Applied Sciences, 29, 38-48.

AN, H. C., SUNG, J. H., LEE, J., SIM, C. S., KIM, S. H. & KIM, Y. 2017. The association between cadmium and lead exposure and blood pressure among workers of a smelting industry: a cross-sectional study. Annals of occupational and environmental medicine, 29, 47.

APANPA-QASIM, A. F., ADEYI, A. A., MUDLIAR, S. N., RAGHUNATHAN, K. & THAWALE, P. 2016. Examination of lead and cadmium in water-based paints marketed in Nigeria. Journal of Health and Pollution, 6, 43-49.

AZIZ, F. H. & RASHEED, R. O. 2017. Heavy metals in water, fishes and sediments in Derbendikhan reservoir, kurdistan Region-Iraq. ZANCO Journal of Pure and Applied Sciences, 29, 19-27.

BADRAN, M., MORSY, R., SOLIMAN, H. & ELNIMR, T. 2018. Assessment of wet acid digestion methods for ICP-MS determination of trace elements in biological samples by using multivariate statistical analysis. Journal of Elementology, 23, 179-189.

BATÁRIOVÁ, A., SPĚVÁČKOVÁ, V., BENEŠ, B., ČEJCHANOVÁ, M., ŠMÍD, J. & ČERNÁ, M. 2006. Blood and urine levels of Pb, Cd and Hg in the general population of the Czech Republic and proposed reference values. International journal of hygiene and environmental health, 209, 359-366.

BUXBAUM, G. 2008. Industrial inorganic pigments, John Wiley & Sons.

CDC. 2013. Fourth National Report on Human Exposure to Environmental Chemicals, Updated Tables. Centers for Disease Control and Prevention National Center for Environmental Health Division of Laboratory Sciences Mail Stop F-20 4770 Buford Highway [Online]. NE Atlanta, p. 30341-3724. Available: https://www.cdc.gov/exposurereport/ [Accessed 10 March 2020].

ČERNÁ, M., SPĚVÁČKOVÁ, V., BENEŠ, B., ČEJCHANOVÁ, M. & ŠMÍD, J. 2001. Reference values for lead and cadmium in blood of Czech population. Int J Occup Med Environ Health, 14, 189-92.

CRINNION, W. J. 2010. The CDC Fourth National Report on Human Exposure to Environmental Chemicals: What it Tells Us About our Toxic Burden and How it Assists Environmental Medicine Physicians. Alternative medicine review, 15.

DECHARAT, S. 2016. Heavy metals exposure and hygienic behaviors of workers in sanitary landfill areas in Southern Thailand. Scientifica, 2016, 1-9.

FORTE, G., MADEDDU, R., TOLU, P., ASARA, Y., MARCHAL, J. A. & BOCCA, B. 2011. Reference intervals for blood Cd and Pb in the general population of Sardinia (Italy). International journal of hygiene and environmental health, 214, 102-109.

HASSAN, M. K., ALFHADY, N. H. & ALJUMAILY, M. A. 2011. Serum heavy metals in patients with fragments and shells of improvised explosive devices. Annals of the College of Medicine Mosul, 37, 8-13.

HEITLAND, P. & KÖSTER, H. D. 2006. Biomonitoring of 37 trace elements in blood samples from inhabitants of northern Germany by ICP–MS. Journal of Trace Elements in Medicine and Biology, 20, 253-262.

HSU, D.-J., CHUNG, S.-H., DONG, J.-F., SHIH, H.-C., CHANG, H.-B. & CHIEN, Y.-C. 2018. Water-based automobile paints potentially reduce the exposure of refinish painters to toxic metals. International journal of environmental research and public health, 15, 899.

ISHOLA, A. B., OKECHUKWU, I. & ASHIMEDUA, U. 2017. Serum level of lead, zinc, cadmium, copper and chromium among occupationally exposed automotive workers in Benin city. Int J Environ Pollut Res, 5, 70-79.

JÄRUP, L. 2003. Hazards of heavy metal contamination. British medical bulletin, 68, 167-182.

JUNAID, M., HASHMI, M. Z. & MALIK, R. N. 2016. Evaluating levels and health risk of heavy metals in exposed workers from surgical instrument manufacturing industries of Sialkot, Pakistan. Environmental Science and Pollution Research, 23, 18010-18026.

JUNAID, M., HASHMI, M. Z., TANG, Y.-M., MALIK, R. N. & PEI, D.-S. 2017. Potential health risk of heavy metals in the leather manufacturing industries in Sialkot, Pakistan. Scientific reports, 7, 1-13.

KAMETI, C. M. 2013. Determination of lead and cadmium levels in decorative paints sold in Nairobi Kenya. Master Thesis, Kenyatta University, Kenya.

KHLIFI, R., OLMEDO, P., GIL, F., FEKI-TOUNSI, M., HAMMAMI, B., REBAI, A. & HAMZA-CHAFFAI, A. 2014. Biomonitoring of cadmium, chromium, nickel and arsenic in general population living near mining and active industrial areas in Southern Tunisia. Environmental monitoring and assessment, 186, 761-779.

KIM, B., YOON, J.-H., CHOI, B.-S. & SHIN, Y. C. 2013. Exposure assessment suggests exposure to lung cancer carcinogens in a painter working in an automobile bumper shop. Safety and health at work, 4, 216-220.

KIM, N.-S. & LEE, B.-K. 2011. National estimates of blood lead, cadmium, and mercury levels in the Korean general adult population. International archives of occupational and environmental health, 84, 53-63.

LADEIRA, C. & VIEGAS, S. 2016. Human Biomonitoring–An overview on biomarkers and their application in Occupational and Environmental Health. Biomonitoring, 3.

LECH, T. 2013. Application of ICP-OES to the determination of barium in blood and urine in clinical and forensic analysis. Journal of analytical toxicology, 37, 222-226.

LENTINI, P., ZANOLI, L., GRANATA, A., SIGNORELLI, S. S., CASTELLINO, P. & DELL'AQUILA, R. 2017. Kidney and heavy metals-The role of environmental exposure. Molecular medicine reports, 15, 3413-3419.

MARTH, E., JELOVCAN, S., KLEINHAPPL, B., GUTSCHI, A. & BARTH, S. 2001. The effect of heavy metals on the immune system at low concentrations. International journal of occupational medicine and environmental health, 14, 375-386.

MIERNA, R., CYNTHIA, N., AFRIYANTI, W., FERRY, C., NEVITA, P. T., DORETHEA, K., MARLISAWATI, S., INDAH, R., AGUS, P. T. & DINA, R. 2015. Biomonitoring for iron, manganese, chromium, aluminum, nickel and cadmium in workers exposed to welding fume: a preliminary study. Russian Open Medical Journal, 4.

MWSTEFA, S. B. & AHMED, I. T. 2019. Effect of Phosphorus Fertilization on Phytoremediation efficacy of Heavy Metals by Wheat and Bean Plants. ZANCO Journal of Pure and Applied Sciences, 31, 18-27.

NAKHAEE, S., AMIRABADIZADEH, A., NAKHAEE, S., ZARDAST, M., SCHIMMEL, J., AHMADIAN-MOGHADAM, J., AKBARI, A., DARMIAN, H. M., MOHAMMADI, M. & MEHRPOUR, O. 2019. Blood lead level risk factors and reference value derivation in a cross-sectional study of potentially lead-exposed workers in Iran. BMJ open, 9, e023867.

OGILO, J., ONDITI, A., SALIM, A. & YUSUF, A. 2017. Assessment of Levels of Heavy Metals in Paints from Interior Walls and Indoor Dust from Residential Houses in Nairobi City County, Kenya. Chemical Science International Journal, 1-7.

OKEWOLE, A. & OMIN, B. 2013. Assessment of Heavy Metal Contents of Some Paints Produced in Lagos, Nigeria. Polytech. J. Sci. Tech, 8, 60-66.

PYSZEL, A., WROBEL, T., SZUBA, A. & ANDRZEJAK, R. 2005. Effect of metals, benzene, pesticides and ethylene oxide on the haematopoietic system. Medycyna pracy, 56, 249-255.

RODRÍGUEZ, J. & MANDALUNIS, P. M. 2018. A review of metal exposure and its effects on bone health. Journal of toxicology, 2018, 10-11.

SABBIONI, E., MINOIA, C., PIETRA, R., FORTANER, S., GALLORINI, M. & SALTELLI, A. 1992. Trace element reference values in tissues from inhabitants of the European Community. II. Examples of strategy adopted and trace element analysis of blood, lymph nodes and cerebrospinal fluid of Italian subjects. Science of the total environment, 120, 39-61.

SANI, A. & ABDULLAHI, I. L. 2017. Evaluation of some heavy metals concentration in body fluids of metal workers in Kano metropolis, Nigeria. Toxicology Reports, 4, 72-76.

SCHULZ, C., WILHELM, M., HEUDORF, U. & KOLOSSA-GEHRING, M. 2011. Update of the reference and HBM values derived by the German Human Biomonitoring Commission. International journal of hygiene and environmental health, 215, 26-35.

SHAFIQUE, U., ANWAR, J. & ALI, S. Z. 2011. Assessment of concentration of lead, cadmium, chromium and selenium in blood serum of cancer and diabetic patients of Pakistan. J. Chem. Soc. Pak, 33, 869.

SIRIVARASAI, J., KAOJAREN, S., WANANUKUL, W. & SRISOMERANG, P. 2002. Non-occupational determinants of cadmium and lead in blood and urine among a general population in Thailand. Southeast Asian Journal of Tropical Medicine and Public Health, 33, 180-187.

SOLENKOVA, N. V., NEWMAN, J. D., BERGER, J. S., THURSTON, G., HOCHMAN, J. S. & LAMAS, G. A. 2014. Metal pollutants and cardiovascular disease: mechanisms and consequences of exposure. American heart journal, 168, 812-822.

STOJSAVLJEVIĆ, A., BORKOVIĆ-MITIĆ, S., VUJOTIĆ, L., GRUJIČIĆ, D., GAVROVIĆ-JANKULOVIĆ, M. & MANOJLOVIĆ, D. 2019. The human biomonitoring study in Serbia: background levels for arsenic, cadmium, lead, thorium and uranium in the whole blood of adult Serbian population. Ecotoxicology and environmental safety, 169, 402-409.

TAHIR, H., ZEB, Q. J. & SULTAN, M. 2010. Assessment of heavy metal exposure around auto body refinishing shops. African Journal of Biotechnology, 9, 7862-7869.

TAYLOR, C. M., EMOND, A. M., LINGAM, R. & GOLDING, J. 2018. Prenatal lead, cadmium and mercury exposure and associations with motor skills at age 7 years in a UK observational birth cohort. Environment international, 117, 40-47.

VITAYAVIRASUK, B., JUNHOM, S. & TANTISAERANEE, P. 2005. Exposure to lead, cadmium and chromium among spray painters in automobile body repair shops. Journal of occupational health, 47, 518-522.

WASOWICZ, W., GROMADZINSKA, J. & RYDZYNSKI, K. 2001. Blood concentration of essential trace elements and heavy metals in workers exposed to lead and cadmium. International journal of occupational medicine and environmental health, 14, 223-229.

WILHELM, M., EWERS, U. & SCHULZ, C. 2004. Revised and new reference values for some trace elements in blood and urine for human biomonitoring in environmental medicine. International journal of hygiene and environmental health, 207, 69-73.

WITTSIEPE, J., FELDT, T., TILL, H., BURCHARD, G., WILHELM, M. & FOBIL, J. N. 2017. Pilot study on the internal exposure to heavy metals of informal-level electronic waste workers in Agbogbloshie, Accra, Ghana. Environmental Science and Pollution Research, 24, 3097-3107.

YOUSEF, S., EAPEN, V., ZOUBEIDI, T., KOSANOVIC, M., MABROUK, A. A. & ADEM, A. 2013. Learning disorder and blood concentration of heavy metals in the United Arab Emirates. Asian journal of psychiatry, 6, 394-400.

ZHANG, L.-L., LU, L., PAN, Y.-J., DING, C.-G., XU, D.-Y., HUANG, C.-F., PAN, X.-F. & ZHENG, W. 2015. Baseline blood levels of manganese, lead, cadmium, copper, and zinc in residents of Beijing suburb. Environmental research, 140, 10-17.