Prospects of Potassium and Phosphate Solubilizing Bacteria for Nodulation Enhancement, growth and yield of Chickpea plant (Cicer arientinum L.)

Kwestan Jawhar  Mustafa; Aras.M. Khudhur

doi:10.21271/ZJPAS.32.5.20

Authors

Kwestan Jawhar Mustafa Department of Soil and Water, College of Agricultural Engineering Science, Salahaddin University-Erbil, Kurdistan Region-Iraq
Aras.M. Khudhur Department of Soil and Water, College of Agricultural Engineering Science, Salahaddin University-Erbil, Kurdistan Region-Iraq

DOI:

https://doi.org/10.21271/ZJPAS.32.5.20

Keywords:

B.circulans, Biofertilizer, Chickpea, K-solubilizing bacteria, P-solubilizing bacteria, Ps.Putida.

Abstract

Seventy one percent of the overall P and K in Erbil governorate soils are in inorganic forms which are unavailable for use in plants. As a result, chemical fertilizer is often used to supplement the nutrient for the growth crops. To reduce the addition of chemical fertilizer to agricultural soils, this research has been conducted to isolate naturally occurring potassium solubilizing microorganisms (KSB) and phosphate solubilizing microorganisms (PSB) from Erbil soil samples and use to improve chickpea growth. Thirty nine efficient PSB isolates were selected based on their ability to clear zone formation on Pikovskaya´s agar medium of and 26 efficient KSB isolates using Aleksandro medium. Depending on microscopical, cultural, and biochemical characteristics, PSB strains belonged to Ps.putida while KSB strains referred to B.circulans. All PSB and KSB strains were screened for their solubilization efficiency on both solid and liquid medium. Bh36 strain of Ps.Putida was the most efficient isolate in P solubilization (94.92%,117.78mg/ml) and the most efficient B.circulans strain in K solubilization was Q1 (87.01%,4.81mg/ml), and they were used for P and K biofertilizers preparation, respectively. Pot experiment showed that seed inoculation with Ps.Putida, B.circulans, and combined (Ps.Putida+ B.circulans) application significantly enhanced chickpea growth. Combined (Ps.Putida+ B.circulans) inoculation recorded the highest increase in shoot height (46.29cm), root length (37.84cm), shoot dry weight (4.28g/plant), number of seed (26.37seeds/plant), number of active nodules (11.56/plant) P (6.35g/plant) and K uptake (65.75g/plant), soil available phosphorus (17.21mg/kg) and soil available potassium (314.5mg/kg). It could be assumed that local Ps.Putida and B.circulans strain can be used as biofertilizer of P and K to improve plant growth and soil fertility.

References

ANJANADEVI, I. P., JOHN, N. S., JOHN, K. S., JEEVA, M. L. & MISRA, R. S. 2016. Rock inhabiting potassium solubilizing bacteria from Kerala, India: characterization and possibility in chemical K fertilizer substitution. Journal of basic microbiology, 56, 67-77.

BADR, M. 2006. Efficiency of K-feldspar combined with organic materials and silicate dissolving bacteria on tomato yield. J Appl Sci Res, 2, 1191-1198.

BAI, Y., ZHOU, X. & SMITH, D. L. 2003. Enhanced soybean plant growth resulting from coinoculation of Bacillus strains with Bradyrhizobium japonicum. Crop science, 43, 1774-1781.

BAKHSHANDEH, E., PIRDASHTI, H. & LENDEH, K. S. 2017. Phosphate and potassium-solubilizing bacteria effect on the growth of rice. Ecological Engineering, 103, 164-169.

BAREA, J.-M., POZO, M. J., AZCON, R. & AZCON-AGUILAR, C. 2005. Microbial co-operation in the rhizosphere. Journal of experimental botany, 56, 1761-1778.

BASAK, B. B. & BISWAS, D. R. 2010. Co-inoculation of potassium solubilizing and nitrogen fixing bacteria on solubilization of waste mica and their effect on growth promotion and nutrient acquisition by a forage crop. Biology and Fertility of Soils, 46, 641-648.

BRENNER, D. J., KRIEG, N. R., STALEY, J. T. & GARRITY, G. M. 2005. Bergey's Manual® of Systematic Bacteriology: Volume Two: The Proteobacteria, Part A Introductory Essays, Springer.

CASANOVA, E., VALDÉS, A. E., FERNÁNDEZ, B., MOYSSET, L. & TRILLAS, M. I. 2004. Levels and immunolocalization of endogenous cytokinins in thidiazuron-induced shoot organogenesis in carnation. Journal of plant physiology, 161, 95-104.

CHAIHARN, M. & LUMYONG, S. 2011. Screening and optimization of indole-3-acetic acid production and phosphate solubilization from rhizobacteria aimed at improving plant growth. Current microbiology, 62, 173-181.

CHITRA, K. & SHARAVANAN, P. 2014. Studies on Potassium Solubilizing Bacteria from Southern Indian Tea Soils. Int. J. Curr. Microbiol. App. Sci, 3, 1045-1052.

DATTA, M., BANIK, S. & GUPTA, R. 1982. Studies on the efficacy of a phytohormone producing phosphate solubilizingBacillus firmus in augmenting paddy yield in acid soils of Nagaland. Plant and Soil, 69, 365-373.

DHANDAPANI, P. 2011. Insoluble phosphate solubilization by bacterial strains isolated from rice rhizosphere soils from Southern India. Int. J. Soil Sci, 6, 134-141.

DOTANIYA, M. 2015. Impact of rising atmospheric CO2 concentration on plant and soil process. Crop growth simulation modelling and climate change. Scientific Publisher, Jodhpur, 69-86.

GLICK, B. R. 1995. The enhancement of plant growth by free-living bacteria. Canadian journal of microbiology, 41, 109-117.

GULL, M., HAFEEZ, F., SALEEM, M. & MALIK, K. 2004. Phosphorus uptake and growth promotion of chickpea by co-inoculation of mineral phosphate solubilising bacteria and a mixed rhizobial culture. Australian Journal of Experimental Agriculture, 44, 623-628.

GUPTA, A., MEYER, J. M. & GOEL, R. 2002. Development of heavy metal-resistant mutants of phosphate solubilizing Pseudomonas sp. NBRI 4014 and their characterization. Current Microbiology, 45, 323-327.

GUPTA, G., PARIHAR, S. S., AHIRWAR, N. K., SNEHI, S. K. & SINGH, V. 2015. Plant growth promoting rhizobacteria (PGPR): current and future prospects for development of sustainable agriculture. J Microb Biochem Technol, 7, 096-102.

HAN, H. & LEE, K. 2005. Phosphate and potassium solubilizing bacteria effect on mineral uptake, soil availability and growth of eggplant. Res J Agric Biol Sci, 1, 176-180.

IAKHONTOVA, L., ANDREEV, P., IVANOVA, M. & NESTEROVICH, L. 1987. BACTERIAL DESTRUCTION OF SMECTITE MINERALS. Doklady Akademii Nauk SSSR, 296, 203-206.

KHAN, M. S., ZAIDI, A., WANI, P. A. & OVES, M. 2009. Role of plant growth promoting rhizobacteria in the remediation of metal contaminated soils. Environmental chemistry letters, 7, 1-19.

KHOSHNAW, M., R.,A., & ESMAIL, A., O., 2020. Using Double Function Solubility Diagram to Study the Effect of Phosphorus Fertilizer on the Availability of Phosphorus in Different Soil Orders. Zanco Journal of Pure and Applied Sciences., 32, 127-136.

KHUDHUR, A. M. 2017. Isolation and characterization of phosphate solubilizing bacteria in Erbil soil and study their effects on Cicer arietinum plant growth and phosphorus uptake. Zanco Journal of Pure and Applied Sciences, 29.

KPOMBLEKOU-A, K. & TABATABAI, M. 1994. Effect of organic acids on release of phosphorus from phosphate rocks1. Soil Science, 158, 442-453.

KUMAR, A., BHARGAVA, P. & RAI, L. C. 2010. Isolation and molecular characterization of phosphate solubilizing Enterobacter and Exiguobacterium species from paddy fields of Eastern Uttar Pradesh, India. African Journal of Microbiology Research, 4, 820-829.

LIU, M., LIU, X., CHENG, B.-S., MA, X.-L., LYU, X.-T., ZHAO, X.-F., JU, Y.-L., MIN, Z., ZHANG, Z. W. & FANG, Y. L. 2016. Selection and evaluation of phosphate-solubilizing bacteria from grapevine rhizospheres for use as biofertilizers. Spanish journal of agricultural research, 14, 26.

MARQUES, A. P., PIRES, C., MOREIRA, H., RANGEL, A. O. & CASTRO, P. M. 2010. Assessment of the plant growth promotion abilities of six bacterial isolates using Zea mays as indicator plant. Soil Biology and Biochemistry, 42, 1229-1235.

MAURYA, B., MEENA, V. S. & MEENA, O. 2014. Influence of Inceptisol and Alfisol’s potassium solubilizing bacteria (KSB) isolates on release of K from waste mica. Vegetos, 27, 181-187.

MEENA, V., MAURYA, B. & BAHADUR, I. 2014a. Potassium solubilization by bacterial strain in waste mica. Bangladesh Journal of Botany, 43, 235-237.

MEENA, V. S., MAURYA, B. & VERMA, J. P. 2014b. Does a rhizospheric microorganism enhance K+ availability in agricultural soils? Microbiological research, 169, 337-347.

MIKHAILOUSKAYA, N. & TCHERNYSH, A. 2005. K-mobilizing bacteria and their effect on wheat yield. Latvian Journal of Agronomy.

MILANI, P. & ANTHOFER, J. 2008. Effect of Azotobacter and Azospirillum on the yield of wheat (Triticum aestivum) and barley (Hordeum vulgare) in Kermanshah and Lorestan, Iran. Improving Water Productivity and Livelihood Resilience in Karkheh River Basin in Iran, 17.

NIANIKOVA, G., KUPRINA, E., PESTOVA, O. & VODOLAZHSKAIA, S. 2002. Immobilizing of Bacillus mucilaginosus--a producer of exopolysaccharides, on chitin. Prikladnaia biokhimiia i mikrobiologiia, 38, 300-304.

O'SULLIVAN, D. J. & O'GARA, F. 1992. Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens. Microbiology and Molecular Biology Reviews, 56, 662-676.

PANHWAR, Q. A., OTHMAN, R., RAHMAN, Z. A., MEON, S. & ISMAIL, M. 2012. Isolation and characterization of phosphate-solubilizing bacteria from aerobic rice. African Journal of Biotechnology, 11, 2711-2719.

PARK, J.-H., LEE, H.-H., HAN, C.-H., YOO, J.-A. & YOON, M.-H. 2016. Synergistic effect of co-inoculation with phosphate-solubilizing bacteria. Korean Journal of Agricultural Science, 43, 401-414.

PEI-XIANG, Y., LI, M., MING-HUI, C., JIA-QIN, X., FENG, H., CHANG-QUN, D., MING-HE, M., DUN-HUANG, F., YAN-QING, D. & FA-XIANG, Y. 2012. Phosphate solubilizing ability and phylogenetic diversity of bacteria from P-rich soils around Dianchi Lake drainage area of China. Pedosphere, 22, 707-716.

PEREIRA, S. I. & CASTRO, P. M. 2014. Phosphate-solubilizing rhizobacteria enhance Zea mays growth in agricultural P-deficient soils. Ecological Engineering, 73, 526-535.

PETTIGREW, W. T. 2008. Potassium influences on yield and quality production for maize, wheat, soybean and cotton. Physiologia plantarum, 133, 670-681.

PLAXTON, W. & LAMBERS, H. 2015. Annual Plant Reviews, Phosphorus Metabolism in Plants, John Wiley & Sons.

PRAJAPATI, K. & MODI, H. 2012. Isolation and characterization of potassium solubilizing bacteria from ceramic industry soil. CIBTech J Microbiol, 1, 8-14.

REHM, G. & SCHMITT, M. 2002. Potassium for crop production. Retrieved February, 2, 2011.

SARIKHANI, M. R., KHOSHRU, B. & GREINER, R. 2019. Isolation and identification of temperature tolerant phosphate solubilizing bacteria as a potential microbial fertilizer. . World Journal of Microbiology and Biotechnology,, 35, 126.

SARKER, A., TALUKDER, N. M. & ISLAM, M. T. 2014. Phosphate solubilizing bacteria promote growth and enhance nutrient uptake by wheat. Plant Science Today, 1, 86-93.

SHAIKH, S. S., SAYYED, R. Z. & REDDY, M. 2016. Plant growth-promoting rhizobacteria: an eco-friendly approach for sustainable agroecosystem. Plant, Soil and Microbes. Springer.

SHENG, X. F. & HE, L. Y. 2006. Solubilization of potassium-bearing minerals by a wild-type strain of Bacillus edaphicus and its mutants and increased potassium uptake by wheat. Canadian journal of microbiology, 52, 66-72.

SHRIVASTAVA, M., SRIVASTAVA, P. & D’SOUZA, S. 2016. KSM soil diversity and mineral solubilization, in relation to crop production and molecular mechanism. Potassium solubilizing microorganisms for sustainable agriculture. Springer.

SHWETHA, M. C. & LAKSHMAN, H. 2013. Effect of AM fungi, Azotobacter and Phosphate solubilizing bacteria in improvement of Amaranthus paniculatus L.-a leafy vegetable. RESEARCH JOURNAL OF BIOTECHNOLOGY, 8, 36-39.

SILINI-CHERIF, H., SILINI, A., GHOUL, M. & YADAV, S. 2012. Isolation and characterization of plant growth promoting traits of a rhizobacteria: Pantoea agglomerans lma2. Pakistan Journal of Biological Sciences, 15, 267.

SINDHU, S., SUNEJA, S., GOEL, A., PARMAR, N. & DADARWAL, K. 2002. Plant growth promoting effects of Pseudomonas sp. on coinoculation with Mesorhizobium sp. Cicer strain under sterile and “wilt sick” soil conditions. Applied Soil Ecology, 19, 57-64.

SINDHU, S. S., DUA, S., VERMA, M. & KHANDELWAL, A. 2010. Growth promotion of legumes by inoculation of rhizosphere bacteria. Microbes for legume improvement. Springer.

SINGH, J. S., PANDEY, V. C. & SINGH, D. 2011. Efficient soil microorganisms: a new dimension for sustainable agriculture and environmental development. Agriculture, ecosystems & environment, 140, 339-353.

TEOTIA, P., KUMAR, V., KUMAR, M., SHRIVASTAVA, N. & VARMA, A. 2016. Rhizosphere microbes: potassium solubilization and crop productivity–present and future aspects. Potassium solubilizing microorganisms for sustainable agriculture. Springer.

WALPOLA, B. C. & YOON, M.-H. 2013. Isolation and characterization of phosphate solubilizing bacteria and their co-inoculation efficiency on tomato plant growth and phosphorous uptake. Afr J Microbiol Res, 7, 266-275.

WU, S., CAO, Z., LI, Z., CHEUNG, K. & WONG, M. H. 2005. Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial. Geoderma, 125, 155-166.

ZHANG, C. & KONG, F. 2014. Isolation and identification of potassium-solubilizing bacteria from tobacco rhizospheric soil and their effect on tobacco plants. Applied Soil Ecology, 82, 18-25.