The Main Bioactive Constituents of Traditional Kurdish Plant Achellia oligocephala DC.; their Antiproliferative and Antioxidant Activities.

Hawraz Ibrahim M. Amin

doi:10.21271/ZJPAS.32.5.10

Authors

Hawraz Ibrahim M. Amin 1Department of Chemistry, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq 2Department of Pharmacy, Paitaxt Technical Institute-Private, Erbil, Kurdistan Region, Iraq

DOI:

https://doi.org/10.21271/ZJPAS.32.5.10

Keywords:

Achellia oligocephala DC., ( ) - Luteolin-6-C-glucoside (isoorientin), Traditional medicinal plant, Antiproliferative and Antioxidant activity.

Abstract

Achellia oligocephala DC. , which grows in the mountains, is very popular as wound healing and gastrointestinal complaints. This research article reports the first study of the phytochemical investigation and biological properties of bioactive secondary metabolites from the traditional Kurdish plant Achellia oligocephala. (+) - Luteolin-6-C-glucoside (AO1) and Lupeol (AO2) were isolated from aerial parts for the first time from this plant. The antiproliferative activity was measured against three human tumour cell lines, MCF7, SkBr3, and BG-1 cancer cells by using the MTT assay. Notably, the (+) - Luteolin-6-C-glucoside showed significant antiproliferative activity against MCF7 cancer cell line, IC₅₀ value (10 µg/mL). The antioxidant activity of AO1 and AO2 were evaluated on total antioxidant capacity (TOAC) test. Interestingly, its showed a remarkable antioxidant activity compared to standard antioxidant. This study confirms that (+) - Luteolin-6-C-glucoside can be considered a natural anticancer and antioxidant compound.

References

ADZU, B., CHINDO, B. A., TARFA, F. D., SALAWU, O. A. & IGOLI, O. J. 2015. Isolation and analgesic property of lupeol from Diospyros mespiliformis stem bark. Journal of Medicinal Plants Research, 9, 813-819.

AGARWAL, R. & RANGARI, V. 2003. Antiinflammatory and antiarthritic activities of lupeol and 19 alpha-H lupeol isolated from Strobilanthus callosus and Strobilanthus ixiocephala roots. Indian Journal of Pharmacology, 35, 384-387.

AL-SNAFI, A. E. 2013. Chemical constituents and pharmacological activities of Milfoil (Achillea santolina)-A Review. International Journal of PharmTech Research, 5, 1373-1377.

AMIN, H. I. M., IBRAHIM, M. F., HUSSAIN, F. H., SARDAR, A. S. & VIDARI, G. 2016. Phytochemistry and ethnopharmacology of some medicinal plants used in the Kurdistan region of Iraq. Natural product communications Journal, 11, 1934578X1601100306.

AMIN, H. I. M., HUSSAIN, F. H. S. & VIDARI, G. V. 2016. Characterization and quantification of some esters of fatty acids from Iris persica L. Bulbs by GC-MS analysis. ZANCO Journal of Pure Applied Sciences, 28, 32-36.

AN, F., WANG, S., TIAN, Q. & ZHU, D. 2015. Effects of orientin and vitexin from Trollius chinensis on the growth and apoptosis of esophageal cancer EC-109 cells. Oncology Letters, 10, 2627-2633.

ANILKUMAR, K., REDDY, G. V., AZAD, R., YARLA, N. S., DHARMAPURI, G., SRIVASTAVA, A., KAMAL, M. A. & PALLU, R. 2017. Evaluation of Anti-Inflammatory Properties of Isoorientin Isolated from Tubers of Pueraria tuberosa. Oxidative Medicine Cellular Longevity, 2017, 5498054.

BRAIEM, R. R., AMIN, H. I. M. & HUSSAIN, F. H. 2017. Free Radical Scavenging Activity of Methanolic Root Extract of Iris postii Mouterde, a Kurdish Herbal Remedy. ZANCO Journal of Pure and Applied Sciences, 29, 146-149.

BÜYÜKOKUROĞLU, M., GÜLÇIN, I., OKTAY, M. & KÜFREVIOĞLU, O. 2001. In vitro antioxidant properties of dantrolene sodium. Pharmacological Research, 44, 491-494.

ÇALıŞ, I., BIRINCIOǦLU, S. S., KıRMıZıBEKMEZ, H., PFEIFFER, B. & HEILMANN, J. 2006. Secondary metabolites from Asphodelus aestivus. Zeitschrift für Naturforschung B, 61, 1304-1310.

CHARISIADIS, P., KONTOGIANNI, V. G., TSIAFOULIS, C. G., TZAKOS, A. G., SISKOS, M. & GEROTHANASSIS, I. P. 2014. 1H-NMR as a structural and analytical tool of intra-and intermolecular hydrogen bonds of phenol-containing natural products and model compounds. Molecules, 19, 13643-13682.

GALLO, M. B. & SARACHINE, M. J. 2009a. Biological activities of lupeol. International Journal of Biomedical and Pharmaciutical Science, 3, 46-66.

GALLO, M. B. & SARACHINE, M. J. 2009b. Biological activities of lupeol. International Journal of Biomedical and Pharmaciutical Science, 3, 46-66.

GARCIA, G. R. M., HENNIG, L., SIELER, J. & BUSSMANN, R. W. 2015. Constituents of Corynaea crassa “Peruvian Viagra”. Revista Brasileira de Farmacognosia, 25, 92-97.

GEETHA, T. & VARALAKSHMI, P. 2001. Anti-inflammatory activity of lupeol and lupeol linoleate in rats. Journal of ethnopharmacology, 76, 77-80.

GILARDONI, G., CHIRIBOGA, X., VITA FINZI, P. & VIDARI, G. 2015. New 3, 4‐Secocycloartane and 3, 4‐Secodammarane Triterpenes from the Ecuadorian Plant Coussarea macrophylla. Chemistry and biodiversity Journal, 12, 946-954.

GÜLÇIN, I., BURSAL, E., ŞEHITOĞLU, M. H., BILSEL, M. & GÖREN, A. C. 2010. Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey. Food and Chemical Toxicology, 48, 2227-2238.

JASH, S. K., GANGOPADHYAY, A., SARKAR, A. & GORAI, D. 2013. Phytochemical investigation of the hexane extract of stem bark of Peltophorum pterocarpum (DC.). Der Pharma Chemica, 5, 49-53.

KIM, A., IM, M., GU, M. J. & MA, J. Y. 2016. Ethanol extract of Lophatheri Herba exhibits anti-cancer activity in human cancer cells by suppression of metastatic and angiogenic potential. Journal of Nutrients Scientific reports, 6, 1-14.

KIM, Y.-H., OH, T. W., PARK, E., YIM, N.-H., PARK, K. I., CHO, W. K. & MA, J. Y. 2018. Anti-inflammatory and anti-apoptotic effects of Acer palmatum Thumb. extract, KIOM-2015EW, in a hyperosmolar-stress-induced In vitro dry eye model. Nutrients, 10, 282.

KONTOGIANNI, V. G., CHARISIADIS, P., PRIMIKYRI, A., PAPPAS, C. G., EXARCHOU, V., TZAKOS, A. G. & GEROTHANASSIS, I. P. J. M. 2013. Hydrogen bonding probes of phenol–OH groups. Organic and biomolecular chemistry Journal, 11, 1013-1025.

KRENN, L., MIRON, A., PEMP, E., PETR, U. & KOPP, B. 2003. Flavonoids from Achillea nobilis L. Zeitschrift für Naturforschung C, 58, 11-16.

KUMAZAWA, T., MINATOGAWA, T., MATSUBA, S., SATO, S. & ONODERA, J.-I. 2000. An effective synthesis of isoorientin: the regioselective synthesis of a 6-C-glucosylflavone. Carbohydrate research Journal, 329, 507-513.

MARCHART, E., HATTENBERGER, A., KRENN, L. & KOPP, B. 2003. Analysis of flavonoids in Achillea nobilis L. bv capillarv electrophoresis. Scientia Pharmaceutica, 71, 133-145.

MORADKHANI, S., KOBARFARD, F. & AYATOLLAHI, S. A. M. 2014. Phytochemical investigations on chemical constituents of Achillea tenuifolia Lam. Iranian journal of pharmaceutical research, 13, 1049.

MOSMANN, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of immunological methods, 65, 55-63.

MÜKEMRE, M., BEHÇET, L. & ÇAKıLCıOĞLU, U. 2015. Ethnobotanical study on medicinal plants in villages of Çatak (Van-Turkey). Journal of ethnopharmacology, 166, 361-374.

NAM, T. G., LIM, T.-G., LEE, B. H., LIM, S., KANG, H., EOM, S. H., YOO, M., JANG, H. W. & KIM, D.-O. 2017. Comparison of anti-inflammatory effects of flavonoid-rich common and tartary buckwheat sprout extracts in lipopolysaccharide-stimulated RAW 264.7 and peritoneal macrophages. Oxidative medicine cellular longevity Journal, 2017.

OLIVEIRA, D. M. D., SIQUEIRA, E. P., NUNES, Y. R. & COTA, B. B. 2013. Flavonoids from leaves of Mauritia flexuosa. Revista Brasileira de Farmacognosia, 23, 614-620.

ŞABANOĞLU, S., KHAZNEH, E., SALTAN, G., TEKIN, M., ERGENE, B. & ACIKARA, Ö. B. 2017. Secondary Metabolites of Achillea sintenisii HUB. MOR. FABAD Journal of Pharmaceutical Sciences, 42, 191-197.

SALEEM, M. 2009. Lupeol, a novel anti-inflammatory and anti-cancer dietary triterpene. Cancer letters, 285, 109-115.

SUN, L., ZHANG, J., LU, X., ZHANG, L. & ZHANG, Y. 2011. Evaluation to the antioxidant activity of total flavonoids extract from persimmon (Diospyros kaki L.) leaves. Food and chemical toxicology, 49, 2689-2696.

UMAMAHESWARI, M. & CHATTERJEE, T. 2008. In vitro antioxidant activities of the fractions of Coccinia grandis L. leaf extract. African Journal of Traditional, Complementary Alternative Medicines, 5, 61-73.