Sex preselection of sheep using mineral solution injection via subcutaneous

Authors

  • Edres Abdulla Hamadamen Department of Animal Resources, College of Agricultural engineering Science, Salahaddin University-Erbil, Erbil, Kurdistan Region, Iraq
  • Abdulqader Ahmed Hussein Department of Animal Resources, College of Agricultural engineering Science, Salahaddin University-Erbil, Erbil, Kurdistan Region, Iraq https://orcid.org/0000-0003-4293-6512

DOI:

https://doi.org/10.21271/ZJPAS.36.6.7

Keywords:

sex preselection, calcium, magnesium, sheep, female.

Abstract

The injection on Calcium (Ca) and Magnesium (Mg) solution is regarded as a method that have been conducted for sex preselection in sheep. Therefore, this trail was aimed to examine the effect of macro minerals such CaMg subcutaneous injected to Kurdish breed ewes during pre and post mating on lamb sex ratio skewing toward female. Thirty ewes at cycling multiparous period (based on their age and live weight (LW)) were randomly divided into two groups (15 ewes per group). Weekly (for four weeks) from four weeks’ days pre- to one week post-mating, each ewe (CaMg group) were subcutaneous injected by 30 ml of CaMg solution or sterile water (control group). Ewes live weigh at the end of the study were numerically higher (2.55 kg) in ewes injected with CaMg compared to control group. The serum ca and Mg were higher (P<0.05) in ewes administrated with CaMg solution than those not administrated. The level of total protein, urea, glucose, and cholesterol at blood serum were not affected (P>0.05) by CaMg injection. Reproductive hormone such estrogen was not different between control and injected ewes (P>0.05). Although, progesterone (at CIDR removal period) were increased (P<0.05) in injected ewes than control ewes. The new female born lambs were 2 times higher (P<0.05) in CaMg group compared to control group. The results of current study confirm that increasing the mineral proportion (Ca and Mg) in the ewes around mating via subcutaneous injection could skew sex ratio toward female in sheep. Thus, the findings of this trial might assist farmers in managing their breeding toward female lambs (which are going to be born in fattening enterprises and male milk production). More research is required to test different methods of altering mineral concentration and investigate the link between minerals in the diet and human sex pre-selection.

References

Abdulkareem, T.A., Eidan, S.M., Shubber, A.M., Ibrahim, F.F., Ali, M.D. and Mohammed, O.A., 2020. Reference Physiological Values in Different Animal Species. Ministry of Agriculture, Iraq, pp.96-97.

Alhimaidi A.R., Aiman A.A., Muath Q.A., Alsaiady M.Y., Amran R.A. and Swelum. A.A, 2021. Sex preselection of sheep embryo by altering the minerals of maternal nutrition. Saudi Journal of Biological Sciences, 28, 680-684.

Arangasamy, A., Selvaraju, S., Parthipan, S., Somashekar, L., Rajendran, D. and Ravindra, J.P., 2015. Role of calcium and magnesium administration on sex ratio skewing, follicular fluid protein profiles and steroid hormone level and oocyte transcripts expression pattern in Wistar rat. Indian Journal of Animal Sciences, 85(11), pp.1190-1194.

Beernink, F.J., Dmowski, W.P. and Ericsson, R.J., 1993. Sex preselection through albumin separation of sperm. Fertility and sterility, 59(2), pp.382-386.

Bicalho, M.L.S., Lima, F.S., Ganda, E.K., Foditsch, C., Meira Jr, E.B.S., Machado, V.S., Teixeira, A.G.V., Oikonomou, G., Gilbert, R.O. and Bicalho, R.C., 2014. Effect of trace mineral supplementation on selected minerals, energy metabolites, oxidative stress, and immune parameters and its association with uterine diseases in dairy cattle. Journal of dairy science, 97(7), pp.4281-4295.

Bolet G., Gueguen L., Dando P. and Ollivier L., 1981. Influence of mineral diet of the sow on the sex ratio of the newborn. Reproduction Nutrition Development, 22, 1073-1081.

Correa SM, Adkins-Regan E, Johnson PA (2005) High progesterone during avian meiosis biases sex ratios toward females. Biology Letters, 1(2):215–218

Gharibi, Z., Shamsolahi, M., Fatahnia, F., Mohammadi, Y. and Shokri, A.N., 2023. Effect of Calcium and Magnesium Supplementation of Ewes during Pre-and Post-Mating on Lamb Sex Ratio. Iranian Journal of Applied Animal Science, 13(1), pp.67-75.

Gould, L. and Kendall, N.R., 2011. Role of the rumen in copper and thiomolybdate absorption. Nutrition Research Reviews, 24(2), pp.176-182.

Hassan, M.G., Chen, C., Ismail, H.A., Zaher, A.R., Cox, T.C., Goodwin, A.F. and Jheon, A.H., 2022. Altering calcium and phosphorus supplementation in pregnancy and lactation affects offspring craniofacial morphology in a sex-specific pattern. American Journal of Orthodontics and Dentofacial Orthopedics, 161(5), pp. e446-e455.

Hussein, A.A., 2017. The effects of forage type and antagonists on copper metabolism in sheep (Doctoral dissertation, Harper Adams University).

James, W.H., 2008. Evidence that mammalian sex ratios at birth are partially controlled by parental hormone levels around the time of conception. The Journal of endocrinology, 198(1), pp.3-15.

Krackow, S. and Burgoyne, P.S., 1997. Timing of mating, developmental asynchrony and the sex ratio in mice. Physiology and behavior, 63(1), pp.81-84.

Machado, V.S., Bicalho, M.L.S., Pereira, R.V., Caixeta, L.S., Knauer, W.A., Oikonomou, G., Gilbert, R.O. and Bicalho, R.C., 2013. Effect of an injectable trace mineral supplement containing selenium, copper, zinc, and manganese on the health and production of lactating Holstein cows. The Veterinary Journal, 197(2), pp.451-456.

Muehleis, P.M. and Long, S.Y., 1976. The effects of altering the pH of seminal fluid on the sex ratio of rabbit offspring. Fertility and Sterility, 27(12), pp.1438-1445.

Naidu, J.S., Arangasamy, A., Backialakshmi, S., Selvaraju, S., Reddy, I.J. and Bhatta, R., 2023. Dietary calcium and magnesium supplemented maternal diets on skewing of sex ratio and sexually dimorphic gene expression in Rabbit (Oryctolagus cuniculus) placenta. The Indian Journal of Animal Sciences, 93(04), pp.372-377.

National Research Council (US). Committee on Nutrient Requirements of Small Ruminants, 2007. Nutrient requirements of small ruminants: sheep, goats, cervids, and new world camelids.

Navara K.J., 2018. Choosing Sexes: Mechanisms and Adaptive Patterns of Sex Allocation in Vertebrates. Springer, Cham, Switzerland

Qoja, A. and Hussein, A.A., 2022. Effect of crude glycerol supplementation via drinking water on lambs performance, some blood haematology, and biochemical metabolites. Zanco Journal of Pure and Applied Sciences, 34(1), pp.110-119.

Rosenfeld, C.S. and Roberts, R.M., 2004. Maternal diet and other factors affecting offspring sex ratio: a review. Biology of reproduction, 71(4), pp.1063-1070.

Saleh D.M. and Iriyanti N., 2011. The effect of adding sodium potassium and calcium-magnesium to the diet of native chicken hen on sex ratio of native chicks. Animal Production, 12, 12- 15

Seki, N., Asano, Y., Ochi, H., Abe, F., Uenishi, K. and Kudou, H., 2013. Reducing effect of calcium in combination with magnesium and lactulose on body fat mass in middle-aged Japanese women. Asia Pacific Journal of Clinical Nutrition, 22(4), pp.557-564.

Spears, J.W., 2003. Trace mineral bioavailability in ruminants. The Journal of nutrition, 133(5), pp.1506S-1509S.

Stolkowski, J. and Lorrain, J., 1980. Preconceptional selection of fetal sex. International Journal of Gynecology and Obstetrics, 18(6), pp.440-443.

Vahidi A.R. and Sheikhha M.H. (2007). Comparing the effects of sodium and potassium diet with calcium and magnesium diet on sex ratio of rat’s offspring. Pakistan Journal of Nutrition, 6, 44-48.

Published

2024-12-31

How to Cite

Edres Abdulla Hamadamen, & Abdulqader Ahmed Hussein. (2024). Sex preselection of sheep using mineral solution injection via subcutaneous. Zanco Journal of Pure and Applied Sciences, 36(6), 61–68. https://doi.org/10.21271/ZJPAS.36.6.7

Issue

Section

Agricultural and Environmental Researches