Detection of Aerolysin Gene in Aeromonas hydrophila from Suspected Farming Fishes (Cyprinus carpio), Erbil Province / Iraq

ibrahim ramadhan ibrahim; Shamall Mohamad Amin Abdullah; Abdulkarim Yasin Karim; Abdulkarim Yasin Karim2

doi:10.21271/ZJPAS.32.1.12

Authors

ibrahim ramadhan ibrahim Department of Fish Recourses and Aquatic Animals, College of Agriculture Salahaddin University-Erbil, Kurdistan Region, Iraq
Shamall Mohamad Amin Abdullah Department of Fish Recourses and Aquatic Animals, College of Agriculture Salahaddin University-Erbil, Kurdistan Region, Iraq
Abdulkarim Yasin Karim Department of Biology, College of science Salahaddin University-Erbil, Kurdistan Region, Iraq
Abdulkarim Yasin Karim2 Department of Biology, College of science Salahaddin University-Erbil, Kurdistan Region, Iraq

DOI:

https://doi.org/10.21271/ZJPAS.32.1.12

Keywords:

Aerolysin, Aeromonas hydrophila, Cyprinus carpio, Polymerase chain reaction.

Abstract

Aeromonas hydrophila consider as the most common pathogenic bacteria which infect many farming fishes and due to many economical losses annually this bacteria found in fresh, brackish waters and ponds. The present study conducted to determine the most reliable method for the diagnosis of A. hydrophila from common carp (Cyprinus carpio), in Agriculture college fish farmduring a farming (May 2017 to October 2018).Results of the present study showed that 60 bacterial samples out of 115 bacterial samples were gram-negative, however 40 samples were motile and only 29 samples showed positive hemolysis on blood agar. Result of molecular diagnosis showed that only 18 bacterial samples have Aerolysin gene (1482 bp).

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