In-vivo effects of Green laser radiation on hematological parameters of albino rats using direct exposure methods.

Abstract

Laser beam has a thermal effect on the exposed body through oscillation of molecules. Rate of the vibrations depend on the time of exposure, intensity of the laser beam, the quantity of the absorbed dose, and the laser wavelength. Impacts of green laser beam on the hematological parameters of Albino Rats investigated in an in-vivo irradiation process. Green laser (200mW, 532nm) was used as a source of non-ionizing radiation to irradiate albino rats, the exposure carried out to heart position (in-vivo) of the rats directly. Fifteen female rats participated. Main blood components: white blood cells (WBC), red blood cells (RBC), and platelet (PLT) counts were evaluated using a direct exposure method for low and high radiation doses. High doses of laser beam affected the WBCs and PLTs significantly (P-value < 0.05), and the low doses were not significant (P-value > 0.05). Laser beam radiation affected on the main blood components at a limited time of exposure. Optimum radiation doses that had high effectiveness on blood component density depended on the type of blood components. As well as, high impacts (high significant) were on the density of PLT not RBCs.

References

FARHAD H M,  JAAFAR M S, ISMAIL A H.( 2011). Control Light Delivery in PDT by Taking Account the Optical Properties of Hair Density on the Skin Surface. Journal of Modern Applied Science. 2 p.325-331.

HUDA K.M., (2017). The effects of laser radiation on viscosity (PCV) of blood. Pak.J.Biotechnol . Vol.14, No.3 , pp.477-479.

ISMAIL A. H., AND ABDULLA K. N., (2021). Biochemical and hematological study of the effects of annual exposure radiation doses on the operators of X-ray and CT-scan in some Erbil hospitals. Radiation Physics and Chemistry, Vol.184, 109466

KUTSCH, V. K. (1993). Laser in Dentistry. Jour. Amer. Dent. Asso. 124: 49-54.

MARKOLF, H. N. (1996). Laser Tissue Interaction. Heidelberg, Germany.

MUSTAFA S. AL MUSAWI, M. S. JAAFAR, B. AL-GAILANI, NASER M. AHMED, FATANAH M. SUHAIMI & NURSAKINAH SUARDI  ,Lasers in Medical Science volume 32, pages405–411(2017).

MUSTAFA S.A., JAFAAR M.S., NASER M. A., AL-GAILANY B.T., FATANAH M. S., 2016. Effects of low power violet laser irradiation on red blood cells volume and erythrocyte sedimentation rate in human blood, Journal of physics; conference series, Vol.1875, 020005-1-020005-7.

RAAD SH. ALNAYLI A., ZAHRAA S. S., FAID SH. H., HADEEL H. S. (2017).The Effect of LASER on Blood Viscosity and Its Influential Relation on the Rapidity of Red Blood Cells Precipitation . American International Journal of Research in Science, Technology, Engineering & Mathematics, 19(1) pp.69- 74.

SITI SAKINA M. F., SUARDI N., MUSTAFA I. S., (2018). In vitro UV-visible spectroscopy study of yellow laser irradiation on human blood. Journal of physics; conference series, Vol.995, 012053.

THAIHAMMER.S, LAHR. A, CLEMENT, SENGEWALD. A, HESKL. W. M, BURGEMEISTER. R, AND SCHUTZE (2003) “Laser Micro tool in Cell Biology Medicine” laser physics ,13,5.

VANENKO, M. & HERING, P. (1998). Hard Tissue Ablation with Mechanically Q – Switched CO2 Laser. SPIE Proc. 3565:110 – 115.

XU Q, MING Z, DART AM, DU XJVAN PELT LF. (2007). Optimizing dosage of ketamine and xylazine in murine echocardiography. Clin Exp Pharmacol Physiol. 4(5-6):499-507.

ZAHRA AL- TIMIMI (2014). Investigating the Effects of Green Laser Irradiation on Red Blood Cells: Green Laser Blood Therapy. Int.Jou. of Applied Research and Studies.Vol.3, Issue 10,pp.1-5.