Direct identification of fungi associated with indoor and outdoor ornamental plants by utilizing PCR assay

Abstract

   Infection by phytopathogenic fungal species can result in significant losses in ornamental plants. For accurate diagnosis of plant diseases, DNA-based techniques have become dependent method. This study aimed to elaborate Polymerase chain reaction (PCR) for rapid detection of fungal pathogens causing diseases on ornamental plants. In this study, specific primers were used to screen suspected plant diseases. The results revealed that Alternaria, Cylindrocladium, Myrothecium, Sclerotium, Cercospora, and Bipolaris, were the most abundant fungi that infect indoor or outdoor ornamental plants. From which, Alternaria alternata, Cylindrocladium sp., and Myrothecium sp. were found to be the most frequent fungi on foliage of these plants.

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